Supplementary Materials for Guigó et al (2003)
PNAS, 100(3):1140-1145 [ Abstract ]
  IMIM * UPF * CRG * GRIB HOME DATASETS Mouse/Human GP Chr19
   
CHROMOSOME 19 RT-PCR SUBMITTED GENES SUMMARY

GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
 
BR
 
HE
 
KI
 
TH
 
LI
 
ST
 
MU
 
LU
 
TE
 
SK
 
EY
 
OV
SEQ
CNF
SEQ
WRG
DIF
SPL
4A4 TS.chr19.6.038  5736182 - 5739887 FWD 1404bp / 2 1 1 1 1 1 0 1 0 1 0 1 1 1 1 0 0
12C4 TS.chr19.6.043  5831872 - 5836735 FWD 1392bp / 7 3 1 0 0 0 0 0 0 1 0 0 1 0 1 0 1
9E2 SGP.chr19_596  26235374 - 26258311 FWD 531bp / 4 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
7G4 TS.chr19.37.007  36218374 - 36231868 FWD 570bp / 7 4 1 1 0 1 1 0 1 1 1 0 1 1 1 0 0
5F4 SGP.chr19_758  37132114 - 37187244 FWD 507bp / 6 5 1 0 1 1 1 0 1 1 1 1 1 1 0 0 0
4A2 TS.chr19.44.022  43791072 - 43804775 REV 1884bp / 7 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
4A3 TS.chr19.48.005  47077273 - 47083529 REV 1041bp / 3 1 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0
11H2 SGP.chr19_1097  53807373 - 53878511 FWD 3420bp / 13 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
1C5 SGP.chr19_1142  56864747 - 56875386 FWD 618bp / 5 2 0 1 0 1 0 0 0 0 0 0 0 1 1 0 0
GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
BR HE KI TH LI ST MU LU TE SK EY OV SEQ
CNF
SEQ
WRG
DIF
SPL
 

TABLE COLUMNS DESCRIPTION

Here we describe what does contain each column in the previous table:

  • Geneva identifier: corresponds to an alphanumeric code for each single gene set of experiments.
  • Predicted gene identifier: the name of the given gene prediction as provided by the software. Both, this column and the previous one, are linked to a single browser page for each gene prediction that contains further information about it.
  • Gene location: the forward coordinates along the chromosome and the corresponding gene strand.
  • Length of the transcript and the number of exons.
  • The selected intron number.
  • Results on tissues: RT-PCR success on each of the mouse tissues tested in this experiment ("green" color meaning success and "red" color for failure). The tissues were: brain (BR), heart (HE), kidney (KI), thymus (TH), liver (LI), stomach (ST), muscle (MU), lung (LU), testis (TE), skin (SK), eye (EY) and ovary (OV).
  • Three extra columns summarizing RT-PCR results:
    • SEQ CNF : RT-PCR succeeded and the sequence of the predicted gene was confirmed.
    • SEQ WRG : the PCR product that was sequenced does not correspond to the predicted one (unspecific amplification).
    • DIF SPL : the RT-PCR determined a different splice site (i.e. an extra exon within the submitted intron).
    Real Positive Genes are those, and only those, having "1" in the "SEQ CNF" column.

 
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