Supplementary Materials for Guigó et al (2003)
PNAS, 100(3):1140-1145 [ Abstract ]
  IMIM * UPF * CRG * GRIB HOME DATASETS Mouse/Human GP Chr18
   
CHROMOSOME 18 RT-PCR SUBMITTED GENES SUMMARY

GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
 
BR
 
HE
 
KI
 
TH
 
LI
 
ST
 
MU
 
LU
 
TE
 
SK
 
EY
 
OV
SEQ
CNF
SEQ
WRG
DIF
SPL
7G3 TS.chr18.8.010  7450418 - 7452522 FWD 321bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
5F2 SGP.chr18_214  16259538 - 16280448 FWD 390bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12H1 TS.chr18.23.008  22563589 - 22588456 FWD 444bp / 4 2 1 1 0 0 0 0 0 0 1 0 0 0 1 0 0
11G3 SGP.chr18_362  27544107 - 27590013 FWD 345bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12G6 TS.chr18.34.010  33452024 - 33458624 FWD 372bp / 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
8H6 SGP.chr18_657  41885906 - 41941681 FWD 357bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
7G2 TS.chr18.55.002  54005448 - 54025342 REV 1095bp / 7 6 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0
5F3 SGP.chr18_854  55826455 - 55837252 REV 384bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12B4 TS.chr18.61.021  60917766 - 60923867 REV 600bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
4A1 TS.chr18.62.026  61794895 - 61831217 REV 2250bp / 14 4 1 1 0 1 1 1 0 0 1 1 1 0 1 0 0
11H5 SGP.chr18_1072  68975067 - 69059418 FWD 570bp / 7 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
5E6 SGP.chr18_1084  70021011 - 70023469 REV 309bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12A2 TS.chr18.75.019  74937526 - 74963546 FWD 474bp / 5 3 1 1 1 1 0 1 0 1 1 1 1 1 1 0 0
8H5 SGP.chr18_1163  76271366 - 76338354 REV 417bp / 5 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12B2 TS.chr18.78.013  77704380 - 77719453 FWD 810bp / 7 5 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0
6G6 SGP.chr18_1261  81957952 - 81985129 REV 216bp / 2 1 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0
1C4 SGP.chr18_1323  85293051 - 85329962 FWD 1584bp / 6 5 1 0 0 0 0 0 1 0 0 0 0 1 1 0 0
5F1 SGP.chr18_1324  85369445 - 85430756 REV 2034bp / 12 7 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0
GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
BR HE KI TH LI ST MU LU TE SK EY OV SEQ
CNF
SEQ
WRG
DIF
SPL
 

TABLE COLUMNS DESCRIPTION

Here we describe what does contain each column in the previous table:

  • Geneva identifier: corresponds to an alphanumeric code for each single gene set of experiments.
  • Predicted gene identifier: the name of the given gene prediction as provided by the software. Both, this column and the previous one, are linked to a single browser page for each gene prediction that contains further information about it.
  • Gene location: the forward coordinates along the chromosome and the corresponding gene strand.
  • Length of the transcript and the number of exons.
  • The selected intron number.
  • Results on tissues: RT-PCR success on each of the mouse tissues tested in this experiment ("green" color meaning success and "red" color for failure). The tissues were: brain (BR), heart (HE), kidney (KI), thymus (TH), liver (LI), stomach (ST), muscle (MU), lung (LU), testis (TE), skin (SK), eye (EY) and ovary (OV).
  • Three extra columns summarizing RT-PCR results:
    • SEQ CNF : RT-PCR succeeded and the sequence of the predicted gene was confirmed.
    • SEQ WRG : the PCR product that was sequenced does not correspond to the predicted one (unspecific amplification).
    • DIF SPL : the RT-PCR determined a different splice site (i.e. an extra exon within the submitted intron).
    Real Positive Genes are those, and only those, having "1" in the "SEQ CNF" column.

 
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