Supplementary Materials for Guigó et al (2003)
PNAS, 100(3):1140-1145 [ Abstract ]
  IMIM * UPF * CRG * GRIB HOME DATASETS Mouse/Human GP Chr17
   
CHROMOSOME 17 RT-PCR SUBMITTED GENES SUMMARY

GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
 
BR
 
HE
 
KI
 
TH
 
LI
 
ST
 
MU
 
LU
 
TE
 
SK
 
EY
 
OV
SEQ
CNF
SEQ
WRG
DIF
SPL
5E5 SGP.chr17_24  4193482 - 4275009 FWD 549bp / 6 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
7F6 TS.chr17.6.019  5922468 - 5932437 FWD 951bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
11G2 SGP.chr17_154  13353117 - 13360009 REV 1482bp / 6 3 1 0 1 1 1 1 0 1 1 1 1 1 1 0 0
7F5 TS.chr17.14.008  13591742 - 13593083 REV 303bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
3H2 TS.chr17.29.002  28063599 - 28091652 FWD 909bp / 7 1 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0
3H3 TS.chr17.31.007  30173756 - 30225745 FWD 3279bp / 19 14 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0
12G1 TS.chr17.38.006  37258665 - 37263411 REV 744bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
3H4 TS.chr17.43.012  42534711 - 42553232 REV 783bp / 4 3 0 0 0 0 0 0 0 1 1 0 0 1 1 0 0
12A4 TS.chr17.49.014  48934357 - 48950556 FWD 750bp / 7 2 1 1 0 0 0 0 0 1 1 0 1 1 1 0 0
1C3 SGP.chr17_1313  58826867 - 58872650 FWD 486bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
8H4 SGP.chr17_1373  63911569 - 63950026 REV 360bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
3H5 TS.chr17.65.006  64362943 - 64379849 REV 372bp / 3 2 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0
3H6 TS.chr17.80.023  79664219 - 79665576 FWD 198bp / 2 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0
7G1 TS.chr17.87.014  86482610 - 86487620 REV 306bp / 3 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0
8C1 SGP.chr17_1732  90329627 - 90340780 REV 372bp / 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
BR HE KI TH LI ST MU LU TE SK EY OV SEQ
CNF
SEQ
WRG
DIF
SPL
 

TABLE COLUMNS DESCRIPTION

Here we describe what does contain each column in the previous table:

  • Geneva identifier: corresponds to an alphanumeric code for each single gene set of experiments.
  • Predicted gene identifier: the name of the given gene prediction as provided by the software. Both, this column and the previous one, are linked to a single browser page for each gene prediction that contains further information about it.
  • Gene location: the forward coordinates along the chromosome and the corresponding gene strand.
  • Length of the transcript and the number of exons.
  • The selected intron number.
  • Results on tissues: RT-PCR success on each of the mouse tissues tested in this experiment ("green" color meaning success and "red" color for failure). The tissues were: brain (BR), heart (HE), kidney (KI), thymus (TH), liver (LI), stomach (ST), muscle (MU), lung (LU), testis (TE), skin (SK), eye (EY) and ovary (OV).
  • Three extra columns summarizing RT-PCR results:
    • SEQ CNF : RT-PCR succeeded and the sequence of the predicted gene was confirmed.
    • SEQ WRG : the PCR product that was sequenced does not correspond to the predicted one (unspecific amplification).
    • DIF SPL : the RT-PCR determined a different splice site (i.e. an extra exon within the submitted intron).
    Real Positive Genes are those, and only those, having "1" in the "SEQ CNF" column.

 
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