Supplementary Materials for Guigó et al (2003)
PNAS, 100(3):1140-1145 [ Abstract ]
  IMIM * UPF * CRG * GRIB HOME DATASETS Mouse/Human GP Chr16
   
CHROMOSOME 16 RT-PCR SUBMITTED GENES SUMMARY

GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
 
BR
 
HE
 
KI
 
TH
 
LI
 
ST
 
MU
 
LU
 
TE
 
SK
 
EY
 
OV
SEQ
CNF
SEQ
WRG
DIF
SPL
1C2 SGP.chr16_38  4289659 - 4321628 FWD 2079bp / 14 8 0 0 0 1 0 0 0 1 1 0 0 0 1 0 0
12C6 TS.chr16.5.024  4668670 - 4678888 REV 1125bp / 8 1 1 1 1 1 1 1 0 1 1 1 1 1 1 0 0
3H1 TS.chr16.28.002  27084340 - 27096839 FWD 459bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12H3 TS.chr16.31.014  30834099 - 30835700 REV 315bp / 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
5E4 SGP.chr16_541  32060479 - 32062179 FWD 204bp / 2 1 0 1 0 0 0 0 1 1 0 1 1 0 1 0 0
11F2 SGP.chr16_651  37766521 - 37823564 FWD 2484bp / 9 4 1 0 0 0 0 1 0 1 1 1 0 0 1 0 0
8H3 SGP.chr16_746  43208879 - 43214635 FWD 459bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
11F3 SGP.chr16_755  43486045 - 43533672 REV 678bp / 4 2 1 1 1 1 1 1 1 1 1 1 1 1 0 1 0
7F4 TS.chr16.45.008  44533203 - 44534946 FWD 303bp / 2 1 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0
8B6 SGP.chr16_836  49512607 - 49597695 REV 510bp / 5 2 0 0 0 0 1 0 0 1 1 0 0 1 0 1 0
8H2 SGP.chr16_1172  78197388 - 78212167 REV 327bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
5E3 SGP.chr16_1411  96547415 - 96571278 REV 150bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
BR HE KI TH LI ST MU LU TE SK EY OV SEQ
CNF
SEQ
WRG
DIF
SPL
 

TABLE COLUMNS DESCRIPTION

Here we describe what does contain each column in the previous table:

  • Geneva identifier: corresponds to an alphanumeric code for each single gene set of experiments.
  • Predicted gene identifier: the name of the given gene prediction as provided by the software. Both, this column and the previous one, are linked to a single browser page for each gene prediction that contains further information about it.
  • Gene location: the forward coordinates along the chromosome and the corresponding gene strand.
  • Length of the transcript and the number of exons.
  • The selected intron number.
  • Results on tissues: RT-PCR success on each of the mouse tissues tested in this experiment ("green" color meaning success and "red" color for failure). The tissues were: brain (BR), heart (HE), kidney (KI), thymus (TH), liver (LI), stomach (ST), muscle (MU), lung (LU), testis (TE), skin (SK), eye (EY) and ovary (OV).
  • Three extra columns summarizing RT-PCR results:
    • SEQ CNF : RT-PCR succeeded and the sequence of the predicted gene was confirmed.
    • SEQ WRG : the PCR product that was sequenced does not correspond to the predicted one (unspecific amplification).
    • DIF SPL : the RT-PCR determined a different splice site (i.e. an extra exon within the submitted intron).
    Real Positive Genes are those, and only those, having "1" in the "SEQ CNF" column.

 
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