Supplementary Materials for Guigó et al (2003)
PNAS, 100(3):1140-1145 [ Abstract ]
  IMIM * UPF * CRG * GRIB HOME DATASETS Mouse/Human GP Chr10
   
CHROMOSOME 10 RT-PCR SUBMITTED GENES SUMMARY

GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
 
BR
 
HE
 
KI
 
TH
 
LI
 
ST
 
MU
 
LU
 
TE
 
SK
 
EY
 
OV
SEQ
CNF
SEQ
WRG
DIF
SPL
1A1 SGP.chr10_27  4935525 - 5206576 REV 18849bp / 98 11 1 1 1 0 0 0 0 1 1 0 1 1 1 0 0
12H2 TS.chr10.10.013  9736293 - 9737890 REV 321bp / 2 1 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0
3B5 TS.chr10.15.005  14358694 - 14360684 REV 984bp / 3 2 1 1 0 0 0 1 0 1 0 1 0 0 1 0 0
7D1 TS.chr10.30.009  29329410 - 29331747 REV 387bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
3B6 TS.chr10.31.004  30169428 - 30172705 REV 258bp / 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0
5A6 SGP.chr10_376  30875302 - 31000710 REV 756bp / 9 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
11A1 SGP.chr10_406  34056523 - 34090538 FWD 447bp / 4 1 0 0 1 1 1 0 1 0 0 0 0 1 1 0 0
1A2 SGP.chr10_503  41578596 - 41591323 FWD 519bp / 6 3 0 0 1 1 1 0 0 1 1 0 1 1 1 0 0
11D6 TS.chr10.42.022  41755268 - 41776052 REV 1023bp / 9 5 1 0 0 0 0 0 0 1 1 1 0 1 1 0 0
3C1 TS.chr10.44.007  43246889 - 43250340 REV 345bp / 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12D6 TS.chr10.45.020  44577543 - 44632081 FWD 825bp / 5 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
8F1 SGP.chr10_589  46312335 - 46357866 FWD 342bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12A3 TS.chr10.49.006  48973463 - 48977720 FWD 744bp / 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
7D2 TS.chr10.72.012  71463852 - 71469963 FWD 381bp / 2 1 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0
3C2 TS.chr10.76.002  75027313 - 75031457 FWD 1497bp / 5 4 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0
3C3 TS.chr10.87.016  86885142 - 86903656 FWD 1620bp / 6 4 0 0 1 0 1 0 0 1 1 0 1 0 1 0 0
5A5 SGP.chr10_1403  92476790 - 92479614 REV 225bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
3C4 TS.chr10.94.005  93205591 - 93212978 REV 624bp / 4 2 1 0 0 0 0 0 0 1 1 0 1 1 1 0 0
3B4 TS.chr10.108.019  107892623 - 107924002 REV 2196bp / 16 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12H5 TS.chr10.118.019  117598175 - 117605235 REV 606bp / 6 3 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0
7C6 TS.chr10.119.010  118823297 - 118826976 REV 402bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
BR HE KI TH LI ST MU LU TE SK EY OV SEQ
CNF
SEQ
WRG
DIF
SPL
 

TABLE COLUMNS DESCRIPTION

Here we describe what does contain each column in the previous table:

  • Geneva identifier: corresponds to an alphanumeric code for each single gene set of experiments.
  • Predicted gene identifier: the name of the given gene prediction as provided by the software. Both, this column and the previous one, are linked to a single browser page for each gene prediction that contains further information about it.
  • Gene location: the forward coordinates along the chromosome and the corresponding gene strand.
  • Length of the transcript and the number of exons.
  • The selected intron number.
  • Results on tissues: RT-PCR success on each of the mouse tissues tested in this experiment ("green" color meaning success and "red" color for failure). The tissues were: brain (BR), heart (HE), kidney (KI), thymus (TH), liver (LI), stomach (ST), muscle (MU), lung (LU), testis (TE), skin (SK), eye (EY) and ovary (OV).
  • Three extra columns summarizing RT-PCR results:
    • SEQ CNF : RT-PCR succeeded and the sequence of the predicted gene was confirmed.
    • SEQ WRG : the PCR product that was sequenced does not correspond to the predicted one (unspecific amplification).
    • DIF SPL : the RT-PCR determined a different splice site (i.e. an extra exon within the submitted intron).
    Real Positive Genes are those, and only those, having "1" in the "SEQ CNF" column.

 
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